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Development of biosensor for protein tyrosine kinases
Author(s) -
Wei Lan-Yi,
Yuan Chiun-Jye
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.770.1
Subject(s) - tyrosine kinase , proto oncogene tyrosine protein kinase src , kinase , biosensor , tyrosinase , biochemistry , tyrosine , protein tyrosine phosphatase , chemistry , receptor tyrosine kinase , phosphorylation , substrate (aquarium) , biology , enzyme , signal transduction , ecology
Protein tyrosine kinase plays an important role in many cellular events, and many diseases are found to be associated with the dysregulation of various protein tyrosine kinases. However, the conventional methods for the detection of the activity of tyrosine kinase were expensive, specific instrument required, and high risk of radioactive contamination. In this study, A simple, quick and label‐free detection method was developed and developed. The general concept of this detection method is based on the fact that tyrosinase‐based amperometric biosensor can catalyze an electrochemical response of tyrosine but not its phosphorylation form, phosphor‐tyrosine. Further studies showed that the tyrosinase‐based biosensor could recognize tyrosyl residue within either dipeptides or heptapeptide p60c‐src substrate I, a specific peptide substrate for Src kinase. The linear dynamic range of detection to p60c‐src substrate I was studied with a sensitivity of 0.71μA μM‐1cm‐2. The effect of various kinase inhibitors in the Src activity was also studied. Financially supporting by NSC‐97‐2311‐B‐009‐001 MY3.