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Interaction of ibogaine with human a3b4 nicotinic receptors in different conformational states
Author(s) -
Emert Ilana,
Targowska-Duda Katarzyna M,
Feuerbach Dominik,
Jozwiak Krysztof,
Arias Hugo R
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.762.1
Subject(s) - phencyclidine , chemistry , nicotinic acetylcholine receptor , nicotinic agonist , stereochemistry , epibatidine , acetylcholine receptor , docking (animal) , radioligand , biophysics , binding site , receptor , biochemistry , nmda receptor , biology , medicine , nursing
The interaction of ibogaine and phencyclidine (PCP) with human (h) α3β4 nicotinic acetylcholine receptors (AChRs) in different conformational states was determined using radioligand binding assays, Ca 2+ influx detections, thermodynamic and kinetic measurements. The results show that ibogaine inhibits (±)‐epibatidine‐induced Ca 2+ influx in hα3β4 AChRs with ~9‐fold higher potency than PCP, [ 3 H]ibogaine binds to a single site in hα3β4 with relatively high affinity ( K d = 0.46 ± 0.006 μM), and ibogaine has slightly higher affinity for desensitized than resting hα3β4 AChRs, compared with PCP. These results correlate with the docking studies suggesting that ibogaine and PCP interact with a binding domain between the serine (position 6′) and valine/phenylalanine (position 13′) rings. This interaction is mediated mainly by van der Waals contacts, which is in agreement with the observed enthalpic contribution determined by non‐linear chromatography. Calculated entropic contribution also indicates local conformational changes. Research support came from the Science Foundation Arizona and Stardust Foundation.