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Complex Peptide Biosensors for Detection of Intracellular Kinase Biomarkers
Author(s) -
Morris May C,
Kurzawa Laetitia,
Van Ngoc,
Pellerano Morgan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.755.1
Subject(s) - cyclin dependent kinase , kinase , intracellular , microbiology and biotechnology , biosensor , peptide , in vitro , cyclin , chemistry , biology , cell cycle , biochemistry , cell
Cyclin‐dependant kinases play a central role in coordinating cell cycle progression, and in sustaining proliferation of cancer cells, thereby constituting attractive pharmacological targets. We have developed a family of environmentally‐sensitive fluorescent peptide biosensors to probe the relative abundance and activities of CDK/cyclin kinases. CDKSENS and CDKACT sensors allow to probe CDK/Cyclin kinases in a sensitive and specific fashion in vitro. Complexation of these peptide biosensors with cell‐penetrating peptide carriers allows to form stable nanoparticles that can probe these kinases in living cells in a non‐invasive fashion (Kurzawa et al. PloS One 2011). This technology can be applied to monitor subtle alterations between healthy and cancer cell lines, to probe kinase levels and activities in tumour biopsies, and to monitor response to therapeutics in vitro and in vivo. Source of Funding: Grants to MCM “Chercheuse d'Avenir” Région Languedoc‐Roussillon and INCA