Premium
Gene Expression Profiling of Oxidative Stress on Vascular Tissue in Puerto Rican Diabetic Patients Undergoing Coronary Artery Bypass Grafting
Author(s) -
Albino-Rodriguez Elinette,
Soltero Ernesto,
Ferder Leon,
Dutil Julie
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.747.1
Subject(s) - medicine , oxidative stress , diabetes mellitus , coronary artery disease , cardiology , artery , type 2 diabetes mellitus , type 2 diabetes , gpx1 , surgery , endocrinology , glutathione peroxidase , catalase
In the US, Puerto Rican Hispanics have the highest prevalence of Type II diabetes. Type II diabetes is the main risk factor for coronary artery disease (CAD), and patients with advanced symptomatic CAD are referred for coronary artery bypass grafting (CABG). High blood sugar causes generation of free radicals that leads to oxidative stress in several tissues. This study aims to evaluate the molecular differences in oxidative stress related gene expression levels in the left internal thoracic artery and saphenous vein conduits of diabetic patients undergoing CABG. RNA extraction and gene expression levels from mammary artery and saphenous vein were compared between diabetic and non‐diabetic patients for 84 genes related to oxidative stress and antioxidant defense. The expression level of the Glutathione peroxidase 1 ( GPX1 ) gene was significantly higher in conduits of diabetic patients with respect to non‐diabetic patients. Our results suggest that oxidative stress modulates certain antioxidant defense in type 2 Diabetes, improving our understanding of the molecular pathways that are modulated in the conduits of diabetic patients undergoing CABG. Research supported by RCMI Grant #5G12RR003050.