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The use of molecular approaches to identify changes in soil microbial communities during storage
Author(s) -
Freeman Victoria Hope,
Patel Niraj,
Larson Sabreena,
Bailey Cheryl
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.742.1
Subject(s) - capillary electrophoresis , microbial population biology , environmental science , soil test , biology , food science , soil science , soil water , genetics , bacteria , microbiology and biotechnology
The purpose of this project is to identify the time interval during storage in which the greatest change in microbial community within soil samples of varying soil types occur. Three storage conditions, air drying, 4° C, and −20°C will be tested, and DNA extracted from stored soil at time intervals of 0, 1, 2, 4, 7, and 14 days. the extracted DNA will undergo PCR to amplify the v3 region of the 16S rDNA. The PCR product will be processed using Capillary Electrophoresis‐ Single Stranded Conformational Polymorphism (CE‐SSCP) on a genetic analyzer. The resulting microbial community fingerprints will be compared using multi‐variant statistical analysis using the StatFingerprint component of the R Software package. Comparisons will be made to determine the time interval that undergoes the most change as well as the storage condition that produced the most change overall. Detailed results will be presented. This project is funded by The NASA Nebraska Fellowship Grant.