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Identification of RNA Polymerase ω subunit in Chlamydia trachomatis
Author(s) -
Allette Kimaada,
Fan Huizhou,
Bao Xiaofeng
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.736.1
Subject(s) - chlamydia trachomatis , rna polymerase , polymerase , protein subunit , biology , recombinant dna , virology , sigma factor , rna , microbiology and biotechnology , enzyme , genetics , gene , biochemistry
The bacteria Chlamydia trachomatis is responsible for sexually transmitted infections. Because of its intracellular developmental process, it poses difficulties for researchers trying to understand it. The goal of this project is to provide evidence that an omega (ω) factor of the RNA polymerase is present in Chlamydia trachomatis . In general, the ω factor aids in the folding of the RNA polymerase's β′ subunit and thus, the assembly of the β′ subunits with α, α, β to form a functional core enzyme in the conformation of α, α, β, β′ and ω. Through BLAST analysis, our lab has found a putative ω subunit in Chlamydia that has a resemblance to E.coli ω subunit. We are in the process of expressing the putative ω with α, β, β′ and σ 66 in E. coli BL21 with or without ω. Whether the protein is true ω or not will be judged by its ability to aid in the yield of the recombinant chlamydial RNA core enzyme.