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Transient exposure to hydrogen peroxide inhibits degradation of phosphorylated IκBα
Author(s) -
Lee Yeji,
Choi Jin,
Jue Dae-myung
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.734.4
Subject(s) - oxidative stress , phosphorylation , iκbα , chemistry , microbiology and biotechnology , nf κb , reactive oxygen species , iκb kinase , kinase , tumor necrosis factor alpha , oxidative phosphorylation , transcription factor , signal transduction , gene , biology , biochemistry , immunology
NF‐κB is a family of transcription factors involved in many cellular functions, such as cell survival, proliferation, chronic inflammation, and stress responses. Reactive oxygen species(ROS) has been implicated as a modulator of NF‐κB activity in various cell types. However the effect of ROS on NF‐κB is controversial. We studied the effect of transient oxidative stress on TNFα‐induced NF‐κB activation in HEK293 cell. Transient exposure of H 2 O 2 caused significant decrease in expression of NF‐κB reporter gene, 15~30 min delay of IκBα degradation and p65 nuclear translocation, but had no effect on IκBα phosphorylation and IκB kinase activity. Expression of NF‐κB reporter gene induced by TNFα was also delayed by H 2 O 2 with a lag of 3 hr compared with TNFα‐stimulated control cell. Our results showed that transient exposure to oxidative stress inhibits degradation of phosphorylated IκBα while it does not modulate IκB phosphorylation by IκB kinase. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology(2011‐0024179)