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The effect of quercitin on oxidative stress‐induced bone‐like cells (UMR 106‐01 BSP)
Author(s) -
McCowan Brandon,
Bria Mariana,
Joseph Eugene E.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.728.5
Subject(s) - quercetin , oxidative stress , viability assay , chemistry , apoptosis , antioxidant , bone cell , flavonoid , pharmacology , medicine , endocrinology , biochemistry
Antioxidants are reported to play a role in bone metabolism, prevention of bone loss, and the reduction of oxidative stress. A reasonable amount of research has been conducted linking quercetin, a flavonoid antioxidant, with treatment of cancer, disorders of skin and connective tissue. However, not much research has been done connecting quercetin with bone health. The purpose of this study was to determine whether quercetin exhibits a protective effect on UMR bone‐like osteosarcoma cells (UMR 106‐01 BSP) by decreasing the effects of oxidative stress in these cells. To test this, UMR cell cultures were co‐treated with differing concentrations of quercetin and 85μM of an oxidant, buthionine sulfoximine (BSO). Caspase‐3 and NF‐ κB (p65 subunit) analyses were performed to measure cell viability and nuclear activity, respectively, using a flow cytometer. F test ANOVA was used to compare variances and all values in the results were expressed as means ±SD. In all cases, p values of <0.05 were considered statistically significant. The results showed that quercetin effectively protected bone‐like cells against the destructive effects of oxidative stress at an optimal concentration very close to 50μM. As quercetin concentrations increased in co‐treated cell cultures, caspase‐3 levels decreased and NF‐κB activity increased. This suggests that quercetin protects bone cells from undergoing apoptosis due to oxidative stress in a dose‐dependent manner by inhibiting caspase‐3. In addition, quercetin may influence the NF‐κB pathway to alter DNA transcription in the nucleus. These findings may help in the elucidation of the potential benefits of quercetin on bone health. (Supported by the Dean's Office of the College of Arts and Sciences of La Sierra University, Riverside, CA).