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Immunohistochemical localization of PACAP and VIP receptors in major salivary glands and the effect of PACAP on saliva secretion in mice
Author(s) -
aka Naoko,
Nakamura Masanori
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.727.1
Subject(s) - vasoactive intestinal peptide , saliva , secretion , submandibular gland , endocrinology , medicine , receptor , substance p , salivary gland , neuropeptide , chemistry , biology
The control of saliva secretion is mainly under the autonomic nervous control. Pituitary adenylate cyclase‐activating polypeptide (PACAP) is now recognized as the multi‐functional neuropeptide in various organs. Vasoactive intestinal polypeptide (VIP) is similar function to PACAP. In this study, we compared the distribution of PACAP receptor, PAC1R and VIP receptor, VPAC1, in major salivary glands; parotid, submandibular and sublingual glands, and examined the effect of PACAP on saliva secretion in 8 weeks old C57BL/6 mice. In parotid gland, PAC1R and VPAC1 were detected in the cells of striated duct. In the case of submandibular gland, PAC1R and VPAC1 were detected in the tall columnar epithelial cells, called pillar cells, in granular ducts and some of the cells in the striated ducts. In sublingual gland, PAC1R and VPAC1 were expressed mainly in the epithelial cells of striated ducts. Intranasal injection of PACAP enhanced the secretion of saliva. Taken together, these results indicated that the distribution of PAC1R and VPAC1 were similar in three major salivary glands and that PACAP and VIP might be the useful agents for the future dry mouth therapuutics.