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The effect of Vivo‐Morpholino targeting Vmat2 on daily physical activity in mouse soleus compared to scrambledmorpholino control
Author(s) -
Ferguson David P,
Schmitt Emily E,
Lightfoot J Timothy
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.716.8
Subject(s) - saline , in vivo , morpholino , western blot , medicine , chemistry , endocrinology , biology , biochemistry , microbiology and biotechnology , zebrafish , gene
We hypothesized that inhibiting Vmat2 would inhibit daily physical activity. We used Vivo‐Morpholinos targeting Vmat2 (Vmat2‐M) and compared those results versus a scrambled morpholino control (SMC) and saline control. All aspects of this study were conducted in conformance with the FASEB Statement of Principles for the use of animals in research and education. At 9 weeks old 18 male C57Bl/6J mice were randomly assigned to a saline (n=6), SMC (n=6), or a Vmat2‐M (n=6) group. After a week of baseline wheel running the mice received an injection of Vmat2‐M (11mg/kg, 110 ul) or saline/SMC(110 ul). After an additional week of wheel running half the cohort was sacrificed to determine Vmat2 knock‐down using western blot. The remaining mice ran for an additional week. There were no differences between saline and SMC in Vmat2 expression (p=0.051). In the Vmat2‐M group, Vmat2 was significantly knocked down compared to the control groups (p=0.018) one week after injection and returned to baseline levels two weeks post injection. Mice receiving Vmat2 injection saw a decrease (p=0.02) in physical activity compared to baseline data. There were no differences in physical activity compared to the control groups. The use of saline or SMC had similar results in this model. Transiently inhibiting Vmat2 in soleus decreased physical activity suggesting a role of Vmat2 in the regulation of physical activity.