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Effects of nordihydroguaiaretic acid and dimethylsulfoxide on experimental cerebral ischemia
Author(s) -
Villanueva Cleva,
Aguilar Jenner,
Guevara-Balcazar Gustavo,
Alva Arturo,
Medina-Santillan Roberto,
Kross Robert David
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.711.14
Subject(s) - ischemia , medicine , anesthesia , cerebral edema , edema , nordihydroguaiaretic acid , dimethyl sulfoxide , ligation , pharmacology , chemistry , biochemistry , organic chemistry , arachidonic acid , enzyme
Cerebral ischemia was produced in male Wistar rats through the permanent ligation of the right carotid and the right middle cerebral arteries. Three groups of rats (n=8) were treated, one hour prior to cerebral ischemia, with: 1) Nothing (control group), 2) An NDGA (10–30 mg/Kg, sc) aqueous solution of dimenthylsulfoxide (DMSO:water 1:3), and 3) DMSO. Eight hours thereafter, the animals were neurophysiologically evaluated and sacrificed by anesthesia overdose. The brain was sliced and incubated in 1% tetrazolium red to evaluate edema and infarct volume. Compared to the control, both the NDGA‐DMSO solution and the DMSO itself significantly decreased edema and infarct volume. Since there was no difference between these treatments, it was concluded that DMSO but not NDGA provided the protection. An additional group was treated with DMSO one hour after cerebral ischemia. The protective effect of DMSO was similar before and after ischemia. This protective effect after ischemia suggests that DMSO could be a useful drug not only for the prevention but also for the treatment of cerebral ischemia. This study was supported by grants SIP20110949, SIP20110954 and CONACYT083090