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Comparative effects of fatty acid synthase inhibitors, C75 and cerulenin, on brown adipose tissue thermogenesis in Wistar rats
Author(s) -
Cassolla Priscila,
Garófalo Maria Antonieta R.,
Kettelhut Isis C.,
Navegantes Luiz C. C.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.705.8
Subject(s) - cerulenin , thermogenesis , brown adipose tissue , medicine , endocrinology , fatty acid synthase , adipose tissue , chemistry , biology , lipid metabolism
Fatty acid synthase (FAS) inhibitors, C75 and cerulenin, are anorexigenic compounds that promote weight loss. In previous studies, we demonstrated that the central injection of C75 increased the sympathetic outflow to brown adipose tissue (BAT) suggesting activation of the non‐shivering thermogenesis. In the present study, we asked whether or not cerulenin, a natural FAS inhibitor, would have similar effects than C75 on BAT. Wistar rats (~230 g) were submitted to BAT sympathetic denervation following by stereotaxic surgery and implant of i.p. or BAT temperature transponders. Seven days later, C75 (150μg/7.5μL), cerulenin (150μg/7.5μL) or RPMI was administered intracerebroventricularly in 24h‐fasted animals. Both C75 and cerulenin increased core body temperature but did not affect the locomotor activity. While the thermal effect of C75 lasted for 9 h (start at 30 minutes of injection), the effect of cerulenin lasted only for 2h (start at 4 h of injection). C75 and cerulenin also increased BAT temperature for 7 h (start at 1 h of injection) and 2 h (after 3 h of injection), respectively. The thermal effects of both FAS inhibitors were abolished by BAT sympathetic denervation. In contrast to C75, cerulenin did not change food intake. In conclusion, the central effects of C75 were faster and stronger than cerulenin on SNS‐activated BAT thermogenesis. Financial support: FAPESP (08/06694‐6)