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Effect of acetylcholinesterase inhibition with pyridostigmine on cardiovascular parameters in mice with myocardial infarction
Author(s) -
Durand Marina Toledo,
Carmo Jussara M.,
Corrêa Wesley G.,
Oliveira Mauro,
Fazan Rubens,
Salgado Helio Cesar
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.703.5
Subject(s) - pyridostigmine , medicine , heart rate , myocardial infarction , anesthesia , occlusion , isoflurane , cardiology , propranolol , basal (medicine) , mean arterial pressure , sham surgery , blood pressure , myasthenia gravis , alternative medicine , pathology , insulin
The benefit of pyridostigmine (PYR), an acetylcholinesterase inhibitor, on mean arterial pressure (MAP), heart rate (HR) and intrinsic heart rate (IHR) was evaluated in mice (C57Bl/6J) submitted to myocardial infarction (MI). Mice were anesthetized (isoflurane) and telemetry transmitters (DSI) were implanted into the left carotid artery. After 5–7 days of recovery, basal MAP (mmHg) and HR (bpm) were recorded for 48 hours (10s every 10min). Further, basal MAP and HR were recorded continuously followed by methylatropine and propranolol administration. After basal recordings, mice were submitted to sham surgery or left anterior descending (LAD) coronary occlusion and received drinking water with or without PYR (3 mg/kg p.o. for 7 days). Five days after LAD occlusion, or sham surgery, recordings were repeated. Sham surgery did not affect MAP, HR and IHR (n=6). LAD occlusion (n= 3) promoted a MI of 11±1 %, decreased MAP (91±1 vs 106±1 before), increased HR (555±11 vs. 481±18 before) and did not affect the IHR (481±11 vs. 466±7 before). LAD occlusion combined with PYR (n=6) did not show any alteration in MAP (101±6 vs. 100±2 before), HR (489±20 vs. 469±15 before) and IHR (448±41 vs. 453±27 before). The results demonstrate that MI caused a decrease in MAP and an increase in HR combined with no alteration in IHR. In addition, PYR prevented the alterations in MAP and HR caused by MI, and did not change the IHR.