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Diesel Exhaust Particulate Matter has both Direct and Indirect Effects on Cardiomyocyte Function
Author(s) -
Gorr Matthew William,
Rodeman James Daniel,
Cormet-Boyaka Estelle,
Wold Loren Eugene
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.697.6
Subject(s) - myocyte , microbiology and biotechnology , diesel exhaust , lung , chemistry , lung function , cell culture , particulates , medicine , endocrinology , biophysics , biology , diesel fuel , genetics , organic chemistry
Exposure to particulate matter (PM) provokes a variety of pathological responses to multiple organ systems. PM particles with a size less than 2.5 μm (PM2.5) have the most physiological relevance, as they are capable of both manifestation into the lung epithelium, and entrance into the bloodstream. Therefore, PM2.5 has the capacity for both direct and lung‐mediated indirect effects on the heart. In the present study, we cultured isolated rat cardiomyocytes with PM2.5 at various concentrations for 3 hours. Additionally, various concentrations of PM2.5 were added to the apical compartment of polarized human bronchial epithelial cells, 16HBE14o‐ to mimic lung exposure. The basal media was collected 24 hours later and used to culture myocytes for 3 hours. Myocytes from both treatments were stimulated in contractile buffer at 1 Hz, and functional analyses were performed using the real‐time IonOptix system. Baseline % Peak Height was significantly reduced with direct PM2.5 culture (7.05±2.07% vs. 4.85±2.68% at 100 μg/ml) and in co‐culture from epithelial cells treated at various concentrations (7.37±3.00% vs. 4.58±2.05 at 100 μg/ml). These results indicate the presence of both direct and indirect effects of PM2.5 on cardiomyocyte function. Future work will elucidate the mechanisms involved in these pathways.