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Glyceollins induce cytoprotective effect by enhancing glutathione synthesis through Nrf2 in hepatoma cells
Author(s) -
Kim Jong-Sang,
Kim Hyo Jung,
Jung Chaelim,
Park In-Sil,
Lim Ji Sun
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.692.2
Subject(s) - glutathione , buthionine sulfoximine , oxidative stress , chemistry , intracellular , antioxidant , microbiology and biotechnology , pharmacology , biochemistry , enzyme , biology
The purpose of this study was to investigate the protective effects of glyceollins against excessive oxidative stress induced by GSH depletion. In mouse hepatoma cells (Hepa1c1c7) subjected to the buthionine sulfoximine (BSO), an inhibitor of gamma‐glutamylcysteine synthetase, the intracellular GSH content was significantly lowered. On the other hand, the incubation with glyceollins in the presence of BSO increased the level of GSH, expression of GSH synthesis enzyme, and nuclear translocation of NF‐E2‐related factor‐2 (Nrf2), compared to the cells treated with BSO only. Nrf2‐antioxidant responsive element (ARE)‐reporter activity assay in HepG2‐C8 showed that BSO increased the ARE‐reporter activity in a dose dependent manner, compared to vehicle‐treated cells, while the cotreatment with glyceollins caused further increase in reporter luciferase activity than BSO alone. Taken together, these results suggest that glyceollins exhibit cytoprotective effects in BSO‐treated cells from oxidative stress by further inducing glutathione synthesis, which was mediated through Nrf2 activation.