Premium
Telemetry‐based oxygen sensor to continuously monitor kidney oxygenation in conscious rats
Author(s) -
Koeners Maarten,
Ow Pei,
Abdelkader Amany,
Verhaar Marianne C.,
Evans Roger G.,
Russell David M.,
Joles Jaap A.,
Malpas Simon
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.690.6
Subject(s) - oxygenation , oxygen , kidney , hypoxia (environmental) , kidney disease , anesthesia , medicine , respiration , acute kidney injury , telemetry , limiting oxygen concentration , chemistry , cardiology , anatomy , organic chemistry , engineering , aerospace engineering
Disturbed kidney oxygenation, i.e. renal hypoxia, may contribute to initiation and progression of both chronic kidney disease and acute kidney injury. A critical barrier to investigate this is lack of methods to chronically measure kidney tissue oxygen. We have developed a novel solution for chronic measurement of oxygen in the kidney. Using a telemetry‐based carbon paste oxygen electrode we continuously recorded kidney oxygenation for 6 weeks in rats, unhindered by anesthesia or restraint. Oxygen values were stable over time with a coefficient of variation (CV) within individual recordings of 16±6% and comparable values between animals; 90±27 μM oxygen. This was in agreement with recordings obtained under anaesthesia with spontaneous respiration; 85±19 μM oxygen and CV: 7±5%. In addition we observed a reproducible response to repeatedly applied changes in inspired oxygen, both in conscious and anesthetized rats. These experiments are the first to monitor renal oxygenation continuously in untethered conscious rats. EU, FP7, Marie Curie Actions, International Outgoing Fellowship.