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In vitro evaluation of anti‐diabetic potential of Piper sarmentosum Roxb. extract
Author(s) -
Krisanapun Chutwadee,
Wongkrajang Yuvadee,
Temsiririrkkul Rungravi,
Phornchirasilp Srichan,
Peungvicha Penchom
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.686.7
Subject(s) - diabetes mellitus , traditional medicine , absorption (acoustics) , chemistry , glucose uptake , food science , medicine , insulin , endocrinology , materials science , composite material
Diabetes is a chronic disease among many people worldwide. The oral anti‐diabetic medications still have side effects, so anti‐diabetic plants are still in research. Piper sarmentosum Roxb., abundance in Asia and traditional usage as remedy for diabetes, is very interesting. The purpose of this study was to evaluate the water extract from aerial part of P. sarmentosum on 2 mechanisms. The yield of the extract was about 16% w/w of dry aerial part. The inhibition of glucose absorption was conducted by the everted sac method using murine intestine. The results revealed that the extract concentration of 5 and 10 mg/ml could significantly lower glucose absorption ( p <0.05) like sodium fluoride, used as positive control, did. The enhancement of glucose utilization was performed using rat diaphragm which represented the muscle cell. Pieces of diaphragm were incubated in Kreb solution with or without the extract for 90 min. The rest of glucose in the media was measured and used for the calculation for muscle glucose consumption. The extract concentration of 2.5–5 mg/ml could enhance glucose consumption about 2–2.5 times in comparison with the control value (without the extract). These results suggest that the hypoglycemic effect of P. sarmentosum may result from the inhibition of glucose absorption and the enhancement of glucose consumption. This work was support by National Research Council, Thailand.