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The effects of melatonin and/or exercise on proliferation of neural stem cell/progenitor cell in focal cerebral ischemic rats
Author(s) -
Lee Minkyung,
Lee Seunghoon,
Lee Youngjeon,
Hong Yonggeun Hong
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.685.20
Subject(s) - melatonin , neural stem cell , neurogenesis , nestin , medicine , progenitor cell , stem cell , endogeny , endocrinology , neuroscience , biology , microbiology and biotechnology
Stroke potently stimulates endogenous neuronal stem cell (ENSC) proliferation and neurogenesis and several factors are released by the ischemic brain. The aim of this study was to determine whether melatonin and/or treadmill exercise could induce proliferation of ENSC, thereby promoting morphological and functional recovery in ischemic brain. Sprague‐Dawley (SD) rats were randomly divided into control, MCAo, MCAo+Exercise (Ex), MCAo+Melatonin (MT), MCAo+Melatonin+Exercise (MT+Ex). Following the MCAo, the 8‐week‐old SD rats were treated twice daily with MT (10 mg/kg) at 7:00 and 19:00, trained twice daily with Ex (20 m/min, 6 days/week) at 17:00 and 22:00. We found that MT+Ex was significantly improved the neurological severity score (p < 0.01), as well as the infarct area (p < 0.01). Dendrite density was noticeably increased in the interventional groups with MT and/or Ex compared to MCAo. We confirmed that MT and Ex were significantly enhanced both Nestin and Foxo 3 mRNA level (p < 0.01). On 3rd, 7th, and 28th day, MT and MT+Ex were significantly increased both DCx and GFAP mRNA level (p < 0.01). In conclusion, we suggest that MT and/or Ex intervention might lead to positive effect on proliferation of ENSCs, thereby promoting morphological reconstruction and subsequently followed by functional recovery in MCAo brain.