Premium
The Investigation of LPA 4 Functions in Zebrafish
Author(s) -
Lin Yu-nung,
Kao Shen-Yung,
Lee Hsinyu
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.683.6
Subject(s) - zebrafish , autotaxin , lysophosphatidic acid , biology , microbiology and biotechnology , receptor , morpholino , phenotype , genetics , gene
Lysophosphatidic acid, LPA, is a structurally simple lysophospholipid. Derived from phospholipid by autotaxin catalyzation, LPA acts as an extracellular signaling molecule activating various receptors, LPA 1–6 . There are pleiotropy characteristics of LPA such as wound healing, cancer progression, cardiovascular function, nervous system regulation, reproduction, platelet activation, hair growth, etc. To clarify the function of different LPA receptors, several knock out animals have been obtained. While autotaxin deficient mouse reveal phenotype as embryonic lethality due to vascular defects, none of the single or double deficient LPA 1–3 mouse observed similar phenotypes. Here, we investigate the forth LPA receptor, LPA 4 , which is structural distinct from LPA 1–3 in zebrafish. We conducted microinjection of LPA 4 morpholino on one cell stage zebrafish embryo. Several phenotypes were observed in LPA 4 knock down zebrafish embryos. During early development, edema around pericardial region was observed, suggesting a vascular leakage or failure of lymphatic vessels to absorb body fluids. Also, a significant of decreasing of heart beats with slowed down of blood flow in some individuals. In addition, slightly decrease of intersegmental vessel numbers reveal abnormality of blood vessel development. We conclude that LPA 4 may regulate blood and lymph vessel development in zebrafish.