Premium
Local adiponectin production in skeletal muscle resistance arteries: effects of exercise and shear stress
Author(s) -
Sapp Glenn Herod,
Chen Bei,
Gurovich Alvaro N.,
Muller-Delp Judy M.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.681.6
Subject(s) - adiponectin , medicine , endocrinology , adipose tissue , skeletal muscle , endothelium , vascular smooth muscle , insulin resistance , obesity , smooth muscle
Adiponectin is as an adipose‐derived secretory protein with anti‐inflammatory and anti‐atherosclerotic effects in the endothelium and vascular smooth muscle. Paradoxically, despite being secreted from adipose tissue, plasma adiponectin correlates inversely with obesity and obesity‐associated cardiovascular disease. The purpose of the current study was to determine whether the endothelium of resistance arteries is a local source of adiponectin. We also investigated the possibility that exercise training upregulates adiponectin in rat skeletal muscle resistance arteries. Real‐time qRT‐PCR was used to assess mRNA for adiponectin in both endothelium‐intact and ‐denuded arteries, and in cultured endothelial cells exposed to shear stress. Adiponectin mRNA was also assessed in skeletal muscle resistance arteries isolated from sedentary and exercise trained rats. Adiponectin mRNA was detected in both intact and denuded arteries. Shear stress increased adiponectin mRNA in cultured endothelial cells, and exercise training increased adiponectin expression in skeletal muscle resistance arteries. These data indicate that both the endothelium and the vascular smooth muscle of skeletal muscle resistance arteries are local sources of adiponectin. Exercise training increases adiponectin expression in skeletal muscle resistance arteries, possibly through a shear stress‐sensitive mechanism.