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Sustained High Luminal Flow In Vivo Decreases Maximal Developed Tension of Bovine Mesenteric Lymphatic Vessels
Author(s) -
Nguyen Tam L,
Dongaonkar Ranjeet M,
Hardy Joanne,
Laine Glen A,
Quick Christopher M,
Stewart Randolph H
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.677.3
Subject(s) - lymphatic system , contractility , lymphatic vessel , isometric exercise , anatomy , in vivo , medicine , mesenteric arteries , cardiology , chemistry , biology , pathology , artery , microbiology and biotechnology , cancer , metastasis
Although lymphatic function is critical to edema resolution within hours or days, most in vitro studies have been focused on the acute effects of transmural pressure and luminal flow on lymphatic contractility. We have shown that lymphatic vessel function adapts to chronic elevation of microvascular pressure; however, the biomechanics of a contracting lymphatic vessel in response to a prolonged increase in luminal flow has not been characterized. Because lymphatic vessel pumping is inhibited by acute increases in flow, we hypothesized that lymphatic vessels exposed to sustained high luminal flow will decrease developed tension. Sustained mesenteric hypertension was created in adult beef cows by occluding a mesenteric vein for 3 days. On day 3, isometric length‐tension relationships of associated mesenteric lymphatic vessels were obtained with a novel tubular organ bath that can maintain both constant luminal volume and luminal flow. While diastolic tension was not different between hypertensive (MVH) and control group (SHAM), there was a significant decrease in systolic tension in MVH at all circumferential lengths. As a result, active tension of MVH was significantly lower than SHAM. We conclude that in response to sustained high luminal flow, lymphatic vessels decrease muscle contractility by decreasing systolic tension without changing diastolic properties. (Supported by NIH and AHA grant)