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Increased Lymphatic Permeability During Shock and Burn Trauma Alters Antigen Presenting Cell Recruitment to Mesenteric Lymph Vessels
Author(s) -
Cromer Walter Emerson,
Zawieja Scott,
Stagg Hayden,
Hunter Felicia,
Tharakan Binu,
Meininger Cynthia,
Childs Ed,
Zawieja David
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.677.11
Subject(s) - lymphatic system , vascular permeability , lymph , lymphatic endothelium , pathology , medicine , immunology
Recent attempts to define mediators of lymphatic permeability and their functional roles have been made but few definitive conclusions have been drawn. We hypothesize that failure of the lymphatic barrier induced by inflammatory cytokines during trauma leads to enhanced trafficking of antigen presenting cells to the lymphatic in the mesentery. Lymphatic endothelial cell (LEC) permeability was measured using a transwell FITC‐BSA assay. LECs were treated with inflammatory cytokines or serum from rats subjected to either hypovolemic shock (HVS) or burn trauma (BT). Also, mesenteric tissues were harvested from sham, HVS and BT and stained for MHCII expression. LEC permeability increased when treated with HVS, or BT serum or inflammatory cytokines (in particular IL‐6, which produced responses similar to HVS & BT). Numbers of MHCII cells associated with mesenteric lymphatics increased with both HVS and BT as expected. We examined the effect of mediators of LEC permeability and what effects this might have on immune cell trafficking. We conclude that LECs can alter their barrier in response to mediators of permeability and that the number of MHCII cells increases around lymphatics under conditions of increased permeability. Together these findings present a new role for lymphatics in both immunology and physiology.