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The effects of circulating angiotensin II levels on vascular gene expression in normotensive rats
Author(s) -
Priestley Jessica,
Fredrich Katherine,
Beyer Andreas,
Lombard Julian
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.675.1
Subject(s) - endocrinology , medicine , angiotensin ii , receptor , endothelin 1 , endothelin receptor , bradykinin , chemistry , gene expression , renin–angiotensin system , angiotensin converting enzyme , messenger rna , gene , biology , blood pressure , biochemistry
Expression of mRNA for 84 genes implicated in hypertension was evaluated using pathway‐focused PCR arrays (SA Biosciences). Comparisons were made between salt‐sensitive (SS) vs. SS.13 BN rats ± angiotensin II (ANG II) infusion (5 ng/kg/min) and in Sprague‐Dawley (SD) rats fed low salt diet (LS), high salt diet (HS), or HS + ANG II. In SS vs. SS.13 BN , significant changes (p < 0.05) were observed for 21 genes with tendency for up‐ or down‐regulation (0.05 < p < 0.10) in 15 others. In SD, expression changes occurred in 16 genes, with trends towards up‐ or down‐regulation in 19 others. Angiotensin converting enzyme (ACE) expression was higher with elevated ANG II levels. Angiotensinogen was down‐regulated in SD rats fed HS but not in SS vs. SS.13 BN . Several genes important to vasoreactivity showed altered expression with variations in circulating ANG II, including those important in the endothelin (ET) system, e.g., the ET B receptor was down‐regulated in HS vs. LS SD and in SS.13 BN vs. SS. In SS.13 BN , ET1 and endothelin converting enzyme 1 were down‐regulated vs. SS. The PGI 2 receptor was down‐regulated in SS vs. SS.13 BN and unchanged in SD. The B2 bradykinin receptor was up‐regulated in HS + ANG II vs. HS SD. These results indicate that plasma ANG II levels affect the expression of genes in cerebral arteries, but there are significant differences in the effect of ANG II on gene expression among strains. [NIH #HL65289 and HL92026].