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Selective Estrogen‐Receptor Modulators Genistein, Resveratrol, and Catechin fail to stimulate the Hepatic Expression of Estrogen‐Responsive Genes Encoding the Avian Apolipoprotein II and Vitellogenin
Author(s) -
Bhatt Vrushank Dharmesh,
Chaudhary Kawshik,
Ariff Ammar Bin,
Boggeti Renuka,
Ratna Warren N
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.673.7
Subject(s) - estrogen , estrogen receptor , vitellogenin , endocrinology , medicine , selective estrogen receptor modulator , resveratrol , antiestrogen , biology , tamoxifen , genistein , chemistry , pharmacology , biochemistry , gene , cancer , breast cancer
Apolipoprotein (apo) II and vitellogenin (VTG) I, II & III are yolk proteins expressed in the avian liver in response to estrogen during egg‐laying. Estrogenic stimulation of the hepatic expression of apo II and VTG is due to both transcriptional increase and mRNA stabilization. We had previously shown the estrogen‐responsive mRNA stabilizing factor, which stabilizes apo II mRNA to be stimulated by estrogen, and selective estrogen receptor modulators (SERMs) such as genistein, resveratrol, tamoxifen, Aroclor, chlordecone (Kepone) and hexachlorophene, but inhibited by clomiphene, toxaphene and bisphenol‐A (Ratnasabapathy et al. 53:1425–1434, 1997, Ratna and Oyemalu, C. J. Steroid Biochem. Mol Biol. 80:383–393, 2002, Ratna, W.N. & Simonelli, Life Sci. 70:1577–1589, 2002; Ratna, W.N. Life Sci 71:865–877, 2002). Reverse Transcription‐Polymerase Chain Reaction (RT‐PCR), Northern blot analysis, RNase protection assays on whole cell RNA and Western blot analysis of cytosolic extracts from livers of roosters treated with estrogen or SERMs such as genistein, resveratrol and catechin showed stimulation of transcription and expression of apo II and VTG I, II & III by estrogen but not the SERMs, demonstrating selective regulation of estrogen‐responsive genes by the latter. This work was supported by science research funds from Arnold and Marie Schwartz College of Pharmacy, Long Island University, NY.