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Anti‐oxidant and anti‐apoptotic effects of fisetin in MPTP‐induced PC12 cells
Author(s) -
Benzeroual Kenza,
Patel Mital
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.672.5
Subject(s) - fisetin , apoptosis , mptp , cytochrome c , chemistry , western blot , pharmacology , oxidative stress , microbiology and biotechnology , biochemistry , biology , medicine , parkinson's disease , disease , flavonoid , antioxidant , gene
Several studies mentioned the association of oxidative stress with Parkinson's disease (PD). This study, investigated the anti‐oxidant and anti‐apoptotic mechanisms of two anti‐inflammatory drugs, Fisetin and Ibuprofen in the MPTP‐injured PC12 cells, a rat model of PD. Previously, we demonstrated that MPTP treatment decreased cell viability, increased NO levels and caspase‐3 activity while pretreatment with fisetin reversed all these effects. To further understand the mechanisms involved, we measured NFkB, pro‐apoptotic Bax, and anti‐apoptotic Bcl‐xL protein expression by western blot analysis, and cytochrome c levels using ELISA. Results showed that fisetin dose‐dependently decreased NFkB expression probably due to decreased NO levels. The study also reported that fisetin decreased Bax and increased Bcl‐XL proteins expression, as well decreased cytochrome c levels. This anti‐apoptotic effect was further confirmed with fluorescence microscopy using a fluorescent PSVue480 probe against apoptotic cells. Ibuprofen effects were more related to anti‐oxidant rather than anti‐apoptotic mechanisms. The present study indicates that fisetin may provide a promising approach for the treatment of neurodegenerative diseases such as PD.