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In‐Vitro and In‐Vivo Studies of Antiasthmatic and Mast Cell Stabilizing Activity of Yemeni Sidr Honey
Author(s) -
Alzubier Aman Ahmad,
Okechukwu Patrick Nwabueze
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.668.1
Subject(s) - histamine , degranulation , chemistry , mast cell , in vivo , pharmacology , bradykinin , in vitro , acetylcholine , contraction (grammar) , immunology , medicine , biology , biochemistry , receptor , microbiology and biotechnology
Yemeni Sidr honey has been reported to possess anti‐inflammatory, antipyretic and analgesic effect. In this study the in‐ vitro and in‐ vivo antiasthmatic and mast cell stabilizing effect of Yemeni Sidr honey were investigated. The inhibition of contraction and hind paw oedema induced by inflammatory mediators such as; histamine, acetylcholine, serotonin (5HT), bradykinin, and leukotriene by Yemeni Sidr honey were examined using guinea pig ileum in organ bath and rat induced paw oedema. Mast cell stabilizing effect was tested, using 0.5 ml subcutaneous injection horse serum along with 0.5ml of triple antigen containing 20000 million microorganisms. Yemini Sidr Honey showed a significant concentration ‐ dependent inhibition of contraction and hind paw oedema induced by the inflammatory mediators and inhibited the degranulation of mast cells. This study revealed that Yemeni Sidr honey possesses significant dose‐dependent antiasthmatic activity and markedly protected the rats’ cells against antigen‐induced challenge of mast cells. This result is in agreement with the previous report and confirms the traditional use.