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ACTION OF HYPERTONIC SALINE SOLUTION (NaCl 7,5%) IN PULMONARY FIBROSIS IN A RODENT MODEL OF ENDOTOXEMIA
Author(s) -
Petroni Ricardo Costa,
Biselli Paolo,
Martins Milton,
Csaba Szabo,
Soriano Francisco
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.658.4
Subject(s) - hypertonic saline , intraperitoneal injection , saline , lipopolysaccharide , pulmonary fibrosis , medicine , fibrosis , endocrinology , pharmacology , chemistry
Hypertonic saline solution (HSS, NaCl 7,5%) has shown to modulates immune function favorably and decrease pulmonary injury triggered by endotoxic shock. Therefore, our objective was to investigate the effects of HSS on the mechanism involved in pulmonary fibrosis, in an experimental model of endotoxemic shock. Wistar rats (220–260 g) received lipopolysaccharide ‐ LPS (10mg/kg, intraperitoneal,ip) and volume after 15 minutes. The animals were assigned in four groups (n=7 per group): control group (not subjected to LPS); LPS group (injected with LPS 10mg/kg i.p); HSS group (injected with LPS and treated with hypertonic saline, 4 mL/Kg) and NS group (injected with LPS and treated with normal saline, 34 mL/kg). At 24h after treatment, pulmonary mechanics, type I collagen expression, metalloproteinase 9 expression and activity, and focal adhesion kinase (FAK) were measured. Normal saline increased pulmonary resistance and elastance, compared to other groups (p<0.05). HSS inhibited collagen expression compared to LPS and NS groups and prevented pulmonary injury by decreased MMP9 activity in tissue (p<0.05). Expression of FAK was decreased in HSS groups compared to LPS and NS groups (P<0.05). Therefore, we concluded that treatment of endotoxemic shock with HSS solution can decreased pulmonary fibrosis and injury through FAK pathway. FAPESP 2009/07478‐8