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Induced myeloperoxidase activity in ovarian cancer mouse model
Author(s) -
Papineni Rao V,
Mor Gil,
McLaughlin William,
Holmberg Jennie,
Craveiro Vinicius
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.657.6
Subject(s) - myeloperoxidase , ovarian cancer , in vivo , tumor microenvironment , intraperitoneal injection , cancer , cancer research , chemistry , tumor progression , medicine , inflammation , pathology , pharmacology , immunology , biology , microbiology and biotechnology
Promotion of neutrophil‐mediated inflammation at the tumor enhances the anti‐tumor immune response. Here we investigated the possibilities of using nutriceuticals in induction of myeloperoxidase (MPO activity) in a tumor mouse model. We monitored in vivo MPO activity in a intraperitoneal ovarian cancer tumor mouse model by injecting (i.p) luminol. Multimodal imaging at different time points were performed to determine both the progression of the intraperitoneal tumor metastasis (fluorescence) and the MPO activity (lumiscence). The results show that basal MPO activity signal was not uniform between the tumors, and in some tumors, the MPO activity was completely absent indicating the presence of a differential tumor microenvironment within the mouse. Enhancement in MPO activity was observed upon administration of a single dose of 0.1 ml (50% volume/volume) of nutriceutical‐ BV9 (i.p injection). BV9 elicited a robust increase in the MPO activity within 3 hrs of its administration and the luminescence signals co localized with the tumors. Further investigations assessing the role of elicited MPO activity in cancer progression will greatly enhance the understanding of the tumor microenvironment and aid in better cancer treatments.