Premium
Human cytomegalovirus and mucoepidermoid carcinoma of salivary glands: cell‐specific localization of active viral and oncogenic signaling proteins
Author(s) -
Melnick Michael,
Sedghizadeh Parish P,
Allen Carl M,
Jaskoll Tina
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.657.14
Subject(s) - human cytomegalovirus , carcinogenesis , mucoepidermoid carcinoma , biology , mapk/erk pathway , salivary gland , cancer research , signal transduction , downregulation and upregulation , neoplastic transformation , cancer , immunology , virus , microbiology and biotechnology , gene , genetics , biochemistry
Human cytomegalovirus (hCMV) infection is common. Given that hCMV is frequently resident in salivary gland (SG) ductal epithelium, we hypothesized that hCMV would be important to the pathogenesis of SG mucoepidermoid carcinoma (MEC). Previously we found that purified CMV induces malignant transformation in SG cells in an in vitro mouse model, and utilizes a pathogenic pathway previously reported for human MEC. Here we present the histologic and molecular characterization of 39 human SG MECs selected randomly from a repository of cases spanning 2004–2011. IHC assays were performed for active hCMV proteins (IE1 and pp65) and the activated COX/AREG/EGFR/ERK signaling pathway. All four prospective causal criteria for viruses and cancer are fully satisfied: (1) protein markers for active hCMV are present in 97% of MECs; (2) markers of active hCMV are absent in non‐neoplastic SG tissues; (3) hCMV‐specific proteins (IE1, pp65) are in specific cell types and expression is positively correlated with severity; (4) hCMV correlates and colocalizes with an upregulation and activation of an established oncogenic signaling pathway (COX/AREG/EGFR/ERK). To our knowledge, this is the first demonstration of hCMV's role in human oncogenesis that fully responds to all of Koch's Postulates as revised for viruses and cancer.