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Lysophosphatidic acid induces reactive oxygen species generation through protein kinase C in PC‐3 prostate cancer cells
Author(s) -
Lin Chu-Cheng,
Lin Chuan-En,
Lin Yueh-Chien,
Lee Hsinyu
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.657.13
Subject(s) - lysophosphatidic acid , reactive oxygen species , cancer research , cell growth , protein kinase c , chemistry , flow cytometry , prostate cancer , cell , cancer cell , cell culture , microbiology and biotechnology , cancer , kinase , medicine , biology , receptor , biochemistry , genetics
Prostate cancer is one of the most frequently diagnosed cancers in males. PC‐3 cell is a popular cell model for investigating late stage prostate cancer behaviors. Lysophosphatidic acid (LPA) is a lysophospholipid regulates multiple cell processes including proliferation, migration and other aggressive cell behaviors in different cancers. In our previous study, we demonstrated that LPA enhances VEGF‐C expression in PC‐3 cell through activating the generation of reactive oxygen species (ROS), which is known to be important in the development of many different cancers. Using flow cytometry, we measured the relative ROS levels in PC‐3 cells. LPA triggers ROS generation within ten minutes. This ROS production can be suppressed by antioxidants, N‐acetylcysteine, TEMPO and Tiron, respectively. By using LPA1/3 antagonist Ki16425, we confirmed that LPA induced ROS is mediated through LPA1/3‐dependent pathway. Moreover, pretreatment with PKC inhibitors can block the ROS generation stimulated by LPA treatment. Overall, we first demonstrated that LPA induces ROS generation in a PKC dependent manner in PC‐3 prostate cancer cell line.