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Effect of conjugated linoleic acid on fatty acid synthesis in soy and coconut oil fed mice
Author(s) -
Sanda Milo W,
Angius Zachary,
Ippagunta Siri M,
Barnes Kimberly M
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.651.6
Subject(s) - conjugated linoleic acid , fatty acid synthase , linoleic acid , acetyl coa carboxylase , coconut oil , fatty acid synthesis , sterol regulatory element binding protein , chemistry , fatty acid , medicine , endocrinology , malic enzyme , lipolysis , enzyme , gene expression , biochemistry , pyruvate carboxylase , biology , adipose tissue , gene , dehydrogenase
Dietary conjugated linoleic acid (CLA) causes a body fat loss, which is enhanced when mice are fed coconut oil (CO), in part due to increased lipolysis. Our objective was to determine the effect of CLA supplementation on the mRNA expression of genes involved in fatty acid synthesis in mice fed soy oil (SO) or CO. Male mice (ICR; n=80; 3 wk‐old) were fed 7% SO or CO diets for 6wk, then 0 or 0.5% CLA for 3,7,10, or 14d. The liver relative mRNA expression of Fatty Acid Synthase (FAS), Acetyl‐CoA Carboxylase (ACC), Malic Enzyme (ME), Sterol Regulatory Element Binding Protein (SREBP), and Stearoyl‐CoA Desaturase (SCD) were determined via real time RT‐PCR and normalized to Acidic Ribosomal Protein. FAS enzyme activity was also determined as NADPH disappearance. CO increased the expression of ME (P < 0.01), FAS (P < 0.001), and SCD (P < 0.001) but decreased the expression of ACC (P < 0.01). CLA increased the expression of ME (P < 0.001). Specifically, the CO+CLA‐fed mice had increased (P < 0.05) ME and SCD mRNA and decreased (P < 0.05) ACC mRNA vs all other diet groups. There was also an effect of day on SCD mRNA, where SCD expression increased (P < 0.01) with increasing days on feed. SREBP mRNA and FAS enzyme activity were not different. It appears that CO‐feeding may cause an increase in fatty acid turnover that could contribute to the greater loss of body fat observed when these mice are fed CLA.