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Maternal Vitamin D Supplementation Results in Higher Expression of Bone Formation Markers at the Growth Plate and Site Specific Effects on Bone Strength in Male Offspring
Author(s) -
Fielding Kristina A,
Chen Jianmin,
Jahani Raha,
Comelli Elena M,
Ward Wendy E
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.650.5
Subject(s) - offspring , endocrinology , weaning , medicine , femur , calcitriol receptor , vitamin d and neurology , tibia , lactation , osteoporosis , vitamin d deficiency , biology , pregnancy , anatomy , surgery , genetics
Lower maternal serum 25‐hydroxyvitamin D or ultraviolet B exposure is associated with lower bone mineral in offspring during childhood. The effect of higher maternal vitamin D status on offspring bone health at adulthood is less clear. Using the CD‐1 mouse model, this study determined if dams fed supplemental (5,000 IU/kg diet) versus low (25 IU/kg diet) levels of vitamin D during pregnancy and lactation affected bone health of male offspring. At weaning, expression of osterix, osteoprotegrin, RANKL and vitamin D receptor (VDR) in the tibia growth plate was measured by immunohistochemical analyses in a subset of male mice (n=8/group). Some offspring were fed their mothers diet or the opposite diet (n=14/group) until age 3 months when bone strength, i.e. peak load, at lumbar vertebra and femur neck and midpoint was measured. At weaning, mice exposed to supplemental vitamin D had higher (p<0.05) expression of osteoprotegrin and osterix and lower (p<0.05) expression of VDR with no difference in expression of RANKL in proliferating or hypertrophic chondrocytes in the tibia growth plate. At 3 months of age, offspring of mothers fed supplemental vitamin D had lower (p<0.05) femur neck peak load with no difference in peak load at other skeletal sites regardless of post‐weaning diet. Further study is required to understand the structural changes that contribute to the lower femur neck peak load.