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Liver antioxidant status after hemorrhage in inbred rat strains fed diets varying in levels of methyl group donors for 9 weeks
Author(s) -
Dubick Michael A,
Rose Rajiv,
Grubbs Dana L,
Barr Johnny L,
Klemcke Harold G
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.648.1
Subject(s) - tbars , antioxidant , glutathione , glutathione peroxidase , medicine , endocrinology , glutathione reductase , superoxide dismutase , chemistry , thiobarbituric acid , catalase , biochemistry , nitric oxide , biology , enzyme , lipid peroxidation
To further characterize epigenetic influences on survival time after hemorrhage (STaH) in rat strains, the present study investigated liver antioxidant status in hemorrhaged BN/Mcwi and DA rats (n=5–12/group) fed synthetic diets deficient (SD), sufficient (SC) or supplemented (SS) with methyl group donors (folic acid, choline, methionine and vitamin B12). Rats were bled 50% of their blood volume and STaH observed up to 240 min. Liver tissue was collected at death and frozen at −80°C until assayed for total antioxidant potential, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), nitric oxide (NO), NAD + and superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase and aconitase activities. Total antioxidant potential was 60% lower and TBARS 2‐fold higher in DA SD‐fed rats compared to the other groups. All antioxidant enzyme activities and levels of GSH, NO and NAD + were also significantly lower (p<0.05) in the SD‐fed rats compared to the SC and SS fed rats. Similar observations were noted in the BN/Mcwi SD fed rats, but changes from their SC and SS groups were of lower magnitude. In addition, STaH was lower (p<0.013) in DA than BN/Mcwi rats for all diets. Taken together these data suggest that putative changes in DNA methylation, subsequent to different methyl group donor diets, can differentially influence liver antioxidant status and STaH in inbred rat strains.