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Regulation of the PBMCs gene expression profile with the Western dietary pattern in healthy men and women
Author(s) -
Bouchard-Mercier Annie,
Paradis Ann-Marie,
Rudkowska Iwona,
Lemieux Simone,
Couture Patrick,
Vohl Marie-Claude
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.647.5
Subject(s) - peripheral blood mononuclear cell , gene expression , ingenuity , microarray , fold change , gene , microarray analysis techniques , medicine , downregulation and upregulation , physiology , biology , immunology , genetics , neoclassical economics , economics , in vitro
Diet regulates gene expression. The objective is to examine gene expression in relation with the Western dietary pattern. Methods 30 Caucasians (13 males; 17 females) were recruited. Dietary patterns were derived from a food frequency questionnaire using factor analysis. RNA was extracted from peripheral blood mononuclear cells (PBMCs), when subjects were in the fasting state. Expression levels of 47 231 mRNA transcripts (> 31000 genes) were assessed using the Illumina Human‐6 v3 Expression BeadChips®. Microarray data was processed with Flexarray software and pathway analysis was assessed using Ingenuity Pathway analysis (IPA). Results The Western pattern was characterised by high intakes of refined grain products and meats. In subjects with high versus low scores for the Western pattern, 193 transcripts were upregulated and 197 were downregulated. IPA reveals that networks related to inflammatory response, immunological diseases and cancer were different in subjects with high versus low scores for the Western pattern. Conclusion Studying gene expression profiles according to dietary patterns may help to understand the overall effect of nutrition in chronic diseases development. CIHR (MOP229488)