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Metabolism of [6]‐shogaol in mice and in cancer cells
Author(s) -
Chen Huadong,
Warin Renaud F,
Sang Shengmin
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.646.19
Subject(s) - chemistry , biotransformation , metabolism , apoptosis , antioxidant , cancer cell , lung cancer , cancer , cell culture , pharmacology , colorectal cancer , biochemistry , biology , medicine , enzyme , genetics
Ginger has received extensive attention because of its antioxidant, anti‐inflammatory, and antitumor activities. However, the metabolic fate of its major components is still unclear. In the present study, the metabolism of [6]‐shogaol, one of the major active components in ginger, in mice and in cancer cells (HCT‐116, HT‐29, H‐1299, and CL‐13), was examined for the first time. Our results indicated that [6]‐shogaol is extensively metabolized in human cancer cells and in mice and the mercapturic acid pathway is one of the major biotransformation pathways of [6]‐shogaol. The major phase I and phase II metabolites of [6]‐shogaol were purified from mouse fecal samples and identified using 1D and 2D NMR spectra as well as tandem mass spectra (MS n , n=2–3). The effects of the major metabolites of [6]‐shogaol on the growth inhibition and induction of apoptosis on human lung and colon cancer cells will also be discussed. This work was supported by NIH Grant CA138277 and CA138277S1 to S. Sang.