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Development and validation of a urinary biomarker for dietary furanocoumarins
Author(s) -
Simpkins Scott William,
Young Lindsay Rae,
Peterson Sabrina
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.646.1
Subject(s) - urine , chromatography , furanocoumarin , chemistry , pharmacokinetics , pharmacology , urinary system , biomarker , food science , medicine , biochemistry
Furanocoumarins from food sources (e.g., parsnip, parsley, citrus) have been shown to inhibit carcinogen‐activating cytochrome P450 enzymes and may therefore play a role in prevention of cancer. Development of a biomarker to assess exposure to dietary furanocoumarins could be an alternative to recall‐based dietary assessment methods and be useful for epidemiologic studies of diet and cancer. In a combined dose‐response and pharmacokinetics study, timed urine samples were collected from 10 men and women over 168 hours following low (2g/kg body weight) and high (4g/kg body weight) doses of furanocoumarin‐containing vegetables (parsnips and parsley). These samples were analyzed to confirm 8‐ methoxypsoralen (8‐MOP) and 5‐methoxypsoralen (5‐MOP) as suitable biomarkers of dietary furanocoumarin intake. Pooled control urine, obtained from subjects following a week‐long abstention from furanocoumarin‐containing foods, was utilized for method development. Following β‐glucuronidase/sulfatase hydrolysis, urine samples were processed via solid phase extraction and analyzed by liquid chromatography‐mass spectrometry. Recoveries of 8‐MOP and 5‐ MOP were > 80%. Intraday coefficients of variation were < 4% for both analytes with estimated detection limits of 6.0 pM (1.3 pg/mL). With analysis times under five minutes, this method is rapid and robust enough for application to future epidemiological studies.