Premium
Eicosapentaenoic acid enhances nitric oxide production through up‐regulating SIRT1 expression in human endothelial cells
Author(s) -
Fukuo Keisuke,
Fukada Rumi
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.644.6
Subject(s) - nitric oxide , eicosapentaenoic acid , chemistry , umbilical vein , gene silencing , nitric oxide synthase , polyunsaturated fatty acid , asymmetric dimethylarginine , biochemistry , microbiology and biotechnology , fatty acid , biology , arginine , in vitro , gene , amino acid , organic chemistry
Accumulating evidence indicates that eicosapentaenoic acid (EPA), a polyunsaturated fatty acid of the n‐3 series extracted from fish oils, has anti‐atherogenic effects. However, the molecular mechanism of these beneficial effects remains unclear. Here, we show that EPA enhances nitric oxide (NO) production from human umbilical endothelial cells (HUVEC) which was determined by a fluorescent indicator DAF‐2. EPA also suppressed an increase in SA‐β‐gal positive cells induced by N G , N G ‐dimethylarginine dihydrochloride (ADMA), a NO synthase inhibitor. siRNA‐mediated SIRT1 gene silencing cancelled EPA‐induced suppression of premature senescence and enhancement of NO production in these cells. These results suggest that up‐regulation of SIRT1 expression in endothelial cells may participate in the mechanism underlying the beneficial effects induced by EPA.