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Ferulic acid enhances γδ‐T cell proliferation
Author(s) -
Rowe Cheryl A,
Bonard Suzanna P,
Stanilka Joy M,
Percival Susan S
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.644.2
Subject(s) - ferulic acid , bran , bioavailability , cell growth , chemistry , in vitro , peripheral blood mononuclear cell , cell , biochemistry , food science , biology , pharmacology , raw material , organic chemistry
A previous human intervention study demonstrated that consumption of wheat bran cereal resulted in an enhancement in γδ‐T cell proliferation ex vivo. This and other studies have suggested that plant bioactive compounds can prime γδ‐T cells, thus increasing their ability to proliferate when stimulated. We asked if ferulic acid, a polyphenol found in high concentration in wheat bran, was able to promote proliferation of these cells in vitro . First, to ask about the bioavailability of ferulic acid from the wheat bran cereal, we simulated digestion in vitro and added the digest to a Caco‐2 cell monolayer. Ferulic acid in the chambers was measured by HPLC analysis. Accumulation in the basolateral chamber and decreases from the apical chamber was both time and dose dependent. Ferulic acid, as a single compound, was added to peripheral blood mononuclear cells and cultured for 24h and 10d in the presence of IL‐2, IL‐15 and PHA‐L. The presence of ferulic acid during culture resulted in an enhanced proliferation of γδ‐T cells in a dose dependent manner. We conclude that ferulic acid is bioavailable from wheat brain and can promote γδ‐T cell proliferation. Supported by Kellogg Corporate Citizen's Fund and AES, UF/IFAS