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Effect of fenugreek methanolic extract in inducing apoptosis through triggering proapoptotic genes
Author(s) -
Alshatwi Ali A,
Hasan Tarique N,
Shafi Gowhar,
Syed Naveed A,
Khoja Kholoud K,
Al-Hazzani Amal A,
Alsaif Mohammed A,
Lei David K. Y.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.644.16
Subject(s) - fadd , apoptosis , tunel assay , pharmacology , caspase 8 , chemistry , caspase 3 , cancer research , traditional medicine , biology , medicine , caspase , programmed cell death , biochemistry
Fenugreek ( Trigonella foenum graecum ) is an active constituent of many traditional medicines to alleviate many diseases including cancer, diabetes, high cholesterol, wounds, inflammation, and gastrointestinal ailments. Since, the mechanism of action of several anticancer drugs is based on their ability to induce apoptosis, the objective of the study was sought to characterize the downstream apoptotic genes regulated by fenugreek methanolic extract (FME) in SiHa cells. FME effectively kills SiHa cells through induction of apoptosis. IC 50 was recorded at 75μg/mL while apoptosis was confirmed by TUNEL assay and q‐PCR assays. Cells were exposed to 75μg/mL FME for 24 and 48 hours, and apoptosis was found in 26.2% and 35.1% cells respectively. Further, expressions of proapoptotic genes e.g. Caspase 3, 8, 9, p53, Fas, FADD, Bax and Bak were studied by q‐PCR. Caspase 3, 8, 9, p53, Bax and Bak were found to be increased significantly whereas expressions Fas and Fadd did not show any significant changes. In summary, the induction of apoptosis by FCE is affected by its ability to increase the expression of pro‐apoptotic genes such as Caspase 3, 8, 9, p53, Bax and Bak and it holds promise for consideration in complementary therapy in cervix cancer patients under chemotherapeutic interventions upon further investigation.