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Electron microscopy study of the alteration of P‐fimbriated E. coli adhesion to human uroepithelial cells
Author(s) -
Kimble Lindsey L,
Mathison Bridget D,
Kaspar Kerrie L,
Khoo Christina,
Chew Boon P
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.644.10
Subject(s) - chemistry , myricetin , escherichia coli , fimbria , proanthocyanidin , cranberry juice , adhesion , food science , biochemistry , microbiology and biotechnology , biophysics , biology , urinary system , quercetin , anatomy , gene , polyphenol , organic chemistry , kaempferol , antioxidant
Prevention of urinary tract infections by cranberry juice is attributed to inhibition of P‐fimbriated E. coli adherence to uroepithelial cells (UEC). This mechanism likely involves competitive binding of papG adhesins by bioactive compounds found in cranberry. We examined the effect of cranberry extract and procyanidins on E. coli fimbriation and adherence to human HTB‐4 UEC. E. coli were pre‐incubated with cranberry extract (2–10 mg PACs/mL), myricetin (0.001–0.01 mg/mL), procyanidin B2 (0.005–0.05 mg/mL) or human urine for 30 min and negatively stained for electron microscopy. Additionally, pre‐incubated E. coli were added to UEC cultures and incubated for 1 h. After washing, UEC were fixed, dehydrated and gold coated for imaging by scanning electron microscopy. E. coli grown on CFA and TSA both produced visible fimbriation; it appears that the fimbrae attached singly or in a branched manner to UEC. Pre‐incubation with cranberry extract, myricetin and procyanidin B2 inhibited E. coli adherence to UEC by altering fimbriae number, structure and distribution. Urine from subjects who consumed cranberry beverage also inhibited E. coli adhesion to UEC compared to subjects given a control beverage. Therefore, cranberry inhibits E. coli adherence to UEC likely by altering bacterial fimbriation. Funded by Ocean Spray Cranberries Inc.