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An improved method for vitamin D3 quantification in cod liver oil supplements
Author(s) -
Scholl Chris,
Metzger Brandon T.,
Barnes David M.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.643.9
Subject(s) - saponification , chromatography , detection limit , chemistry , extraction (chemistry) , vitamin , cod liver oil , high performance liquid chromatography , solid phase extraction , sample preparation , solvent , matrix (chemical analysis) , food science , biochemistry
Sample preparation for the determination of vitamin D typically involves saponification and/or liquid/liquid solvent extraction. Cod liver oil has substantial amounts of vitamin D and has a traditional use as a vitamin D supplement, however, its oily sample matrix creates difficulty in extraction and remaining lipid residues limit UV detection. Conventional quantification requires two HPLC columns and runs (normal phase preparative and reverse phase analytical) or recently expensive mass spectrometers to measure vitamin D. Here we describe a method using normal and reverse phase SPE to achieve good cleanup of residual lipids. The complete serving size of eight commercially available cod liver oil supplements were prepared using a 2g NH2 SPE column followed by 500mg C‐18 column before analytical quantification. Using this method, a limit of detection of 20IU/serving was achieved. This method provides a simpler alternative for vitamin D detection in cod liver oil samples using SPE and a single HPLC run with UV detection and may have additional use in the quantification vitamin A and tocopherols.