Premium
Iron overload upregulates the expression of regenerating isletderived family genes in rat pancreas
Author(s) -
Coffey Richard,
Nam Hyeyoung,
Knutson Mitchell
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.641.25
Subject(s) - pancreas , gene expression , islet , gene , microarray , downregulation and upregulation , biology , microarray analysis techniques , beta cell , endocrinology , medicine , messenger rna , cell , diabetes mellitus , microbiology and biotechnology , biochemistry
Iron overload is associated with an increased incidence of hyperglycemia and diabetes resulting from beta cell destruction. The aim of the present study was to identify genes that are regulated by iron in the pancreas. Sprague‐Dawley rats were fed modified AIN‐93G diets that were either iron deficient, adequate, or overloaded for 3 wk. Microarray analysis of RNA isolated from pancreas revealed that the most upregulated mRNAs by iron overload were the regenerating islet‐derived genes (Reg) Reg1a, Reg3a, and Reg3b, which are associated with islet cell regeneration and diabetogenesis. Quantitative RT‐PCR analyses of these genes confirmed that their mRNA levels were more than 20 fold higher in iron overload relative to controls. These data suggest that Reg mRNAs may be sensitive indicators of pancreatic beta cell stress during iron overload. To investigate the effect of iron per se on Reg gene expression, INS1 cells, a rat pancreatic beta cell line, were incubated with or without 200 μM Fe‐NTA for 48 h. We found that Reg mRNA expression was unaffected by iron, suggesting that iron loading of beta cells is not responsible for elevated Reg expression in iron‐loaded pancreas. Funded by NIH. Grant Funding Source : NIH