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Dose‐Response Effect of Long‐Term Quercetin Supplementation on Metabolomics and Quercetin Conjugate Profile in Adults
Author(s) -
Cialdella-Kam Lynn,
Nieman David C.,
Sha Wei,
Meaney Mary Pat,
Knab Amy M.,
Shanely R. Andrew
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.637.4
Subject(s) - quercetin , chemistry , metabolite , glucuronide , pharmacology , isorhamnetin , urine , placebo , chromatography , oxidative stress , antioxidant , medicine , endocrinology , biochemistry , kaempferol , alternative medicine , pathology
Quercetin, a flavonol in fruits and vegetables, has been demonstrated to have antioxidant, anti‐inflammatory, and immunomodulating influences. Using a randomized double‐blinded placebo‐controlled trial, we examined if 2 different quercetin doses (500 or 1000 mg/d) would alter metabolic profiles and serum quercetin conjugate levels in adults (58 women and 42 men; 40–83y). Overnight fasted blood samples were collected at 0‐, 1‐, and 3‐mo and analyzed for metabolic profiles using 2 mass spectrometry platforms (UPLC‐QTOFMS and GC‐TOFMS) with partial least square discriminant analysis (PLS‐DA) and linear mixed model with repeated measures. After accounting for age, sex, and BMI, quercetin supplementation was associated with significant shifts in 163 metabolites/quercetin conjugates (FDR p‐value <0.05). The top 5 metabolite shifts were an increase in serum guaiacol, 2‐oxo‐4‐methylthiobutanoic acid, allocystathionine, and two bile acids. Inflammatory and oxidative stress metabolites were not affected. PLS‐DA revealed a clear separation only between the 1000 mg/d and placebo groups (Q2Y=0.763). The quercetin conjugate, isorhamnetin‐3‐glucuronide, had the highest concentration at 3‐mo followed by quercetin‐3‐glucuronide, quercetin‐3‐sulphate, and quercetin diglucuronide respectively. In humans, quercetin exerts disparate and wide‐ranging metabolic effects especially with 1000 mg/d dose.