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rLosac Induces Pro‐survival Mechanisms In Dorsal Root Ganglia Neurons
Author(s) -
Alvarez-Flores Miryam Paola,
Remuzgo Cesar Manuel,
Cury Yara,
Chudzinski-Tavassi Ana Marisa
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.611.6
Subject(s) - apoptosis , viability assay , microbiology and biotechnology , mtt assay , biology , cell growth , embryonic stem cell , cell , biochemistry , gene
Losac (a protein from Lonomia obliqua caterpillar) shows high similarity with the four first Ig‐like domains of NCAMs from human which are crucial for embryonic development of neural system. Previous studies known that Losac is capable to inhibit endothelial cell death. Using a model of serum deprivation, we investigated the molecular mechanism by which recombinant Losac (produced in bacteria), exerts its protective effect in dorsal root ganglia (DRG) neurons. Cell survival (MTT assay) was better observed after 48h of treatment. Immunoblot analysis showed an increase in phospho‐p44/42 MAPKs after 6h of treatment with Losac. It was observed a reduction of apoptotic DNA and the increasing of the number of cells in the S phase (48 and 72h). In addition, it was observed an increase of the mitochondrial membrane potential (rhodamine‐123) and KI‐67 proliferation marker. All these results indicate that rLosac trigger cell viability through the activation of survival pathways in the early hours of cell contact with Losac and subsequent DNA synthesis and proliferation. It will be further investigated the potential application of Losac on neurodegenerative diseases. Support: FAPESP, CAT‐CEPID.