Premium
Temporal hierarchy of membrane restructuring during cold acclimation in fish
Author(s) -
Ward Kyle,
Williams E Eugene
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.600.1
Subject(s) - restructuring , fish <actinopterygii> , abundance (ecology) , acclimatization , phospholipid , messenger rna , hierarchy , enzyme , biology , chemistry , microbiology and biotechnology , membrane , biochemistry , ecology , gene , political science , fishery , law
Climate change may have dramatic affects on the diversity and abundance of fish. It has been shown fish can restructure membrane phospholipids to aid in their survival in response to a change in water temperature. Biochemical evidence suggests these changes do not occur at the same time or rate. Some reactions occur rapidly, whereas others are delayed. We are currently trying to elucidate this hierarchy for mRNA expression for reactions involved in membrane restructuring. We have developed primers for many of the enzymes involved in these restructuring pathways including de novo phospholipid synthesis, in situ phospholipid modifications and phospholipid head group alterations. Our goal is to develop additional real time probes based on PCR products obtained from primers designed for enzymes in these restructuring pathways. To date, five such probes have been developed and preliminary data show that changes in mRNA expression are occurring at different rates. These data will help us further understand temperature acclimation of fish at the molecular level. This work is supported by the Department of Biological Sciences and the Henson School of Science and Technology at Salisbury University.