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Formulation Development of Alcohol‐Free Oil‐in‐Water Microemulsions as Potential Adjuvants and Vehicles for Vaccines
Author(s) -
Muderhwa Jean Mulimbi
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.597.7
Subject(s) - microemulsion , pulmonary surfactant , chemistry , glycerol , adjuvant , chemical engineering , chromatography , mineral oil , emulsion , organic chemistry , biochemistry , medicine , engineering
An alcohol‐free, oil‐in‐water microemulsion was formulated with mineral oil and Span80/Tween 60 (25/75, HLB 12.29) as surfactant system. Using glycerol as co‐surfactant, the amount of water solubilized depended on the surfactant system to glycerol ratio, the oil chain length, the oil content and the salinity of the water. With this system, one can formulate an isotropic, shear thinning dispersion containing 20% (w/w) of mineral oil and up to 25% (w/w) of water at surfactant to glycerol ratio of 0.50. Interestingly, the inclusion of prototypical model antigens, such as conalbumin and lysozyme, at immunizing dose of 0.025 mg/ml did not affect the formation or the stability of the microemulsions. Likewise, addition of aluminum adjuvants, such as aluminum hydroxide and aluminum phosphate, at a dose of 1 mg/ml did not have an effect either. Overall, the results suggest that these dispersions have the potential of being versatile adjuvants and carriers for vaccine in a composite adjuvant formulation and useful experimental tools with which to investigate the role of the surface in antigen recognition and adjuvanticity due to their large surface area as opposed to emulsions.