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Chemical approaches to the investigation of protein‐membrane binding interactions using synthetic lipid probes
Author(s) -
Best Michael D.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.595.1
Subject(s) - phosphatidic acid , diacylglycerol kinase , membrane , microbiology and biotechnology , cell membrane , cell , chemistry , membrane protein , membrane lipids , signal transduction , cell surface receptor , function (biology) , biochemistry , cell signaling , biology , phospholipid , protein kinase c
Signaling lipids present in cell membranes act as important regulators of biological processes and have been implicated in the onset of numerous diseases. A common role of these lipids is the recruitment of proteins to the cell membrane surface through binding events that generally regulate protein function and localization. While understanding these interactions at the molecular level is of great interest, such efforts are hindered by the complexity of the membrane environment in which binding occurs. Towards this end, chemical strategies will be presented by which protein–membrane recognition events can be efficiently characterized. First, the design and synthesis of lipid probes corresponding to signaling lipids including diacylglycerol, phosphatidic acid and the phosphoinositides will be described. Next, the application of these probes for elucidating binding details will be presented, including the development of multi‐format microarray analysis for investigating protein–lipid recognition in different contexts, and bifunctional probes that allow for collective labeling of target receptors in cell extracts, including those associated with disease.