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Functionalized‐single‐walled nanotube (f‐SWNT)‐assisted in vitro delivery of the oncogene suppressor genes to cancer cells
Author(s) -
Radominska-Pandya Anna,
Karmakar Alokita,
Bratton Stacie M,
Xu Yang,
Mahmood Meena,
Ghosh Anindya,
Biris Alexandru S
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.580.8
Subject(s) - in vitro , cancer cell , gene delivery , suppressor , in vivo , cancer , plasmid , gene , oncogene , dna , cell , cancer research , apoptosis , microbiology and biotechnology , chemistry , biology , genetic enhancement , cell cycle , biochemistry , genetics
In this work, we have examined the possibility of delivering f‐SWNT‐DNA complexes containing p53 genes into MCF‐7 breast cancer cells. The major goal of these studies has been to develop protocols for efficient DNA binding and delivery into cancer cells by using the well‐characterized p53 gene as a model for cancer cell destruction. These studies have resulted in the successful generation of f‐SWCNTs with circular plasmid DNA attached to the outer surface, which, when introduced in vitro to cancer cell cultures, were found to induce apoptosis in a significantly larger percentage of the cells as compared to the nanotubes without plasmid DNA attached to their outside surface. The methods presented here can now be applied as delivery platforms for other genes of interest for various biomedical applications. We hypothesize that specific tumor suppressor genes can be specifically targeted to cancer cells using nanoparticles both in vitro and in vivo and can become an effective tool in cancer treatment. (NIH‐ GM075893 to AR‐P; ASTA 08‐CAT‐03 to ASB).