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Identification of the Differentiation Status of Individual Hematopoietic Cells from Mouse Bone Marrow using Secondary Ion Mass Spectrometry
Author(s) -
Kraft Mary L,
Frisz Jessica F.,
Choi Ji Sun,
Wilson Robert L.,
Harley Brendan A.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.579.5
Subject(s) - haematopoiesis , lymphopoiesis , bone marrow , stem cell , microbiology and biotechnology , progenitor cell , biology , phenotype , immunology , chemistry , biochemistry , gene
The ability to self‐renew and differentiate into multiple types of blood and immune cells renders hematopoietic stem cells (HSCs) of significant value for tissue engineering applications. To enable engineering stem cell fate within laboratory settings, the combination of chemotactic and micromechanical properties that direct HSC maturation must be elucidated. This task requires determining the differentiation stage of individual cells within ex vivo culture systems. New experimental approaches are required to identify the differentiation stage of individual cells and to correlate this information on maturation stage with cell location within their environment. Here we demonstrate that multivariate analysis of time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) data can be used to successfully classify individual hematopoietic cells from mouse bone marrow according to their distinct lymphopoiesis phenotype, specifically HSCs, common lymphoid progenitors, or mature B cells. This research was supported by a Career Award at the Scientific Interface from the Burroughs Wellcome Fund.