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Cloning and expression of Cryptochrome proteins from Arabidopsis thaliana
Author(s) -
Velez Julian D.,
Dyer James H.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.576.4
Subject(s) - cryptochrome , arabidopsis thaliana , arabidopsis , biology , cloning (programming) , circadian clock , function (biology) , flavin group , microbiology and biotechnology , biochemistry , botany , gene , mutant , enzyme , computer science , programming language
Two cryptochrome proteins, CRY1 and CRY2, occur in plants and animals. CRY1 regulates the circadian clock in a light‐dependent fashion in insects and plants. Also in plants, blue light photoreception can be used to cue developmental signals. Despite much research on the topic, the specific way that the pterin and flavin chromophores function in these proteins in Arabidopsis thaliana is still poorly understood. The goal of this research was to clone and express the Arabidopsis CRY1 and CRY2 proteins in an E. coli expression system. The constructs were cloned into a pBAD‐His vector and purified using Ni‐NTA affinity chromatography. Purified proteins will be used for structural and functional analysis.